Análise in sílico de domínios proteicos ligantes de cobre do complexo paracoccidioides

Abstract

The fungi of the genus Paracoccidioidessp are etiologic agents of systemic disease paracoccidioidomycosis (PCM) that first affects the lungs by inalation of spores of the fungus and can spread to several organs. In the infectious process the pathogen expresses some proteins in order to capture nutrients from the medium, which are necessary for its survival. Therefore, the objective of this work is the in silico analysis of copper protein domains in the Paracoccidioides complex. The copper binding proteins of the Paracoccidioides complex were obtained through the public domain, the Cu-protein sequences were obtained through the Broad Institute database and pedant3 database, in silico analysis was performed by Sequence Manipulation Suite software after obtaining of protein domains. The analysis of domains revealed a total of 1148 sequences in which 664 belonged to P. lutzii and 484 to P. brasiliensis. In addition, among these, domains rich in histidine, cysteine and methionine.