Rastreamento de interações intermoleculares da proteína triose fosfato isomerase do fungo patogênico Paracoccidioides brasiliensis

Abstract

Paracoccidioides brasiliensis is a thermodimorphic fungus and the causative of paracoccidioidomycosis, systemic mycosis of more prevalence in the Latin American. The fungus infects the host by pathway respiratory. The process of adhesion of fungus to the lung epithelium is essential to the establishment of infection and is mediated by protein interactions. The triosephosphate isomerase of Paracoccidioides brasiliensis is a protein presents in the cytoplasm and wall cellular the fungus that has function of adhesion. This work evaluated the protein interactions of this protein with cellular extract of fungus Paracoccidioides brasiliensis and mouse lung using the technique of pull-down. We identified two heat shock proteins of yeast fase of fungus that interacted with the triosephosphate isomerase, an Hsp70 and Hsp60.